Mouse Transforming Growth Factor Beta 3 (TGFb3) ELISA Kit is for quantitative detection of activated mouse TGF-beta 3 in cell culture supernates, serum, plasma (EDTA) and urine. Strip well format. Reagents for up to 96 tests.
The mouse TGF-beta 3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TGF-beta 3 has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF-beta 3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-BiotinPeroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TGF-beta 3 amount of sample captured in plate.
More Details:
- Detection Target: Natural and recombinant Mouse TGF-beta 3
- Reactivity: Mouse
- Range: 31.2pg/ml-2000pg/ml
- Sensitivity: < 10 pg/ml
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Please note – this product replaces our old product number IRKTAH5450 (same product, new number).
